Sandeep Arora
Chitkara University, India
Title: Formulation and evaluation of Herbal extract coated pellets (HDT) with anti-inflammatory and hepatoprotective activity for Prophylactic daily use as food supplements
Biography
Biography: Sandeep Arora
Abstract
With increasing incidences of hepatitis, fibrosis and cirrhosis due to lifestyle changes, food habits, drug adverse effects, alcoholic and fatty damage, it is relevant that focus should now be on prophylactic and preventive measures, to take care of day to day factors causing cellular injury and inflammatory processes. Traditional foods and traditional therapeutic systems the world over including Ayurveda point to the benefits of regular herbal intake, which indirectly provided a prophylactic antiinflammatory activity and show antiarthritic action.
Slurry comprising herbal extracts was prepared by suspending in a solution of the excipients, dissolved or suspended in sufficient water and lsopropyl alcohol (IPA) to make the slurry sprayable. After milling through a machine adapted for grinding suspension in order to reduce the particle size of extract, the suspension was applied on the excipient pellets in the classic pharmaceutical fluidized bed coating device, which consists simply of a vertical cylinder with an air-permeable bottom and an upward spraying nozzle close above the bottom, or a downward-spraying nozzle mounted above the product mass. The temperature of the fluidizing air was balanced against the spray rate to maintain the mass of pellets at the desired level of moisture and stickiness while the coating was built up. A finishing layer over the extract layer of 1 % of an anti- static ingredient such as talc or silicon dioxide, simply dusted on the surface of the pellets and other coats were applied.
The pellets were made palatable and coloured with colourants and flavours added to increase patient compliance for daily intake as prophylactic food, and optimized for uniformity of content, dissolution and moisture content, and then for anti-inflammatory and hepatoprotective activity. The Hepatoprotective pellet (HDT) treatment stimulated hepatoprotective effects that were proven by attenuating serum AST and ALT activities. The antioxidant activities of the HDT pellets can ameliorate oxidative stress contributing to the amount of intracellular antioxidant enzymes, which was confirmed by GSH and CAT assay. Centrilobular necrosis, lymphocytes infiltration and steatosis were apparent in CCl4-treated group, whereas administration of the HDT significantly reverses these abnormal indexes. This indicates that HDT can ameliorate oxidative stress to preserve hepatic function and hepatic injury induced by CCl4.